Cell lines are used mainly in biomedical studies. Cell Line Authentication is a crucial tool used widely in various fields such as drug discovery, genetics, toxicity testing, medicine, vaccine development, cell biology, and reconstructive medications. Each cell line has a unique feature, which is utilized in different studies.
Why is a Cell Line Authentication Test Required?
Over the past years, genetic researchers have discovered that their decades-long research has failed only due to the lack of an accurate Cell Line Authentication DNA test in India. Often, a cell line of interest is misidentified at the origin. If not found in time, an investigator would have continued using the same cell line for his investigation and may never get the expected results. It is a common issue while working with cell lines.
The second issue with cell line authentication is that they may be cross-contaminated, where the contaminant can be a different cell from other samples, environmental pollutants, etc.
Third, the most crucial issue with a cell line is that the original cell line may become genetically drifted over time. In such matters, the cells may lose their identity and become different cells by genetic alteration. Experts and researchers acknowledge that the latter is more harmful than the first two kinds of errors, as one may be unaware that the cells are gradually changing and may continue getting false results over time.
Need for Cell Line Authentication DNA Test in India
- A cell line authentication test is required whenever a new cell line is established. It should also be performed before freezing when the line is unstable.
- The cells must be checked in the condition of whether more than one cell line is handled in a lab to evade cross-contamination.
- The cells must also be reviewed before running a new investigation.
- ATCC/NCBI/NIH/ICLAC suggests validating cell lines for journals and grant applications.
Process Used for Cell Line Authentication DNA Test in India
The extensive use of cells for investigation and industrial purposes such as cell genomics, biology, and proteomics has raised the possibility of cross-contamination of cell cultures. The cell line authentication test helps to recognize the relevance of the query cell line with the reference standard, cross-contamination in the cell line, and the gender of a query cell line.
Short Tandem Repeat (STR) DNA typing produces reproducible data that comply with guidelines set by leading scientific organizations. STRs are repeating and non-coding sequences of the DNA which are studied in this method. The STRs are amplified using multiplex PCR (polymerase chain reaction) which makes multiple copies of the STRs. Then using capillary electrophoresis, these STRs are separated to be investigated later using GMIDX software.
Why Choose DNA Forensics Laboratory for a Cell Line Authentication DNA Test in India?
DNA Forensics Laboratory Pvt. Ltd. is among the best DNA testing companies in India, providing accredited POM (peace of mind) DNA tests and NABL-accredited Organ Transplant Tests. It also offers embassy-approved DNA Immigration tests and accurate Cell Line Authentication DNA tests in India.
They provide comprehensive cell line authentication services using STR Profiling. Furthermore, they offer accurate, reliable, reasonably priced, and fast human cell line authentication DNA test services in India.
Listed below are some of their esteemed clients:
- IIIM Jammu and IIT Kanpur
- All India Institute of Medical Science (AIIMS)
- Bhabha Atomic Research Institute (BARI)
Other important aspects that make them best are:
- They have advanced and latest methodologies for the test.
- Their testing facility is accredited by the National Accreditation Board for Testing and Calibration Laboratories (NABL).
- Fastest turnaround time of 10-15 working days.
- Highly skilled scientists and a team of technical experts carry out each DNA test with utmost precision and accuracy.
DNA Forensics Laboratory Pvt. Ltd. 