Advantages of Cell Line Authentication in Scientific Research

Cell lines, derived from animal or human tissues, are invaluable tools in scientific research. Cell lines have revolutionized fields like cancer research, drug development, and genetics, offering consistent and reproducible results. Cultivated under specific conditions, they serve as models for understanding biological processes and diseases. Cell lines are fundamental in studying complex cellular mechanisms, offering insights that drive medical innovations and contribute significantly to scientific knowledge, ultimately shaping the future of healthcare and biotechnology. This article will explore the errors and advantages of Cell Line Authentication DNA tests in India.

Errors Detected in Cell Line Authentication Test

• Cross-Contamination: Cross-contamination between cell lines can occur during culturing, handling, or processing of samples. To prevent cross-contamination, labs should follow strict protocols for cell culture, including regular testing, etc., to maintain the authenticity of cell lines.

• Misidentification: Misidentification of errors occurs when a cell line is incorrectly identified or labeled. It may involve using the wrong source for a cell line. Moreover, it can result from wrong record-keeping or laboratory mix-ups.

• Genetic Drift: Cell lines can undergo genetic changes over time due to prolonged culture or suboptimal handling. Furthermore, cells may lose their uniqueness and become different types of cells of genetic alteration.

How Does Cell Line Work?

Cell line authentication is conducted through short tandem repeats. Short tandem repeats are repetitive sequence elements that are three to six base pairs long. These sequences are polymorphic, and a specific locus pattern can be repeated at any time.

The ATCC/ANSI/ASN-0002 has mentioned short tandem repeat profiling as the best human cell line authentication process. First, STRs are amplified by polymerase chain reaction using primers outside the repeat sequence. The resulting profile is isolated using capillary electrophoresis to specify the number of repetitions in the sample. The profile is then compared with a reference sample to confirm the origin of the cell line. 

Benefits of Getting Cell Line Authentication DNA Test in India

Cell line authentication tests offer several advantages, particularly in the realm of scientific research and medical advancements. Here are some key benefits of cell line authentication DNA tests in India:

• Maintaining Research Integrity: Authenticating cell lines ensures the precision and dependability of research results. This helps maintain research integrity and minimizes the risk of publishing inaccurate findings.

• Preventing Cross-Contamination: Cell line authentication testing helps identify cross-contamination, where cells from one line contaminate with another. Preventing and detecting such contamination is vital for proper experimental results.

• Supports Drug Development: Using authenticated cell lines is vital for drug discovery and testing in pharmaceutical research. It ensures that the results accurately represent the effect of potential drugs on the intended cell type.

• Quality Control in the Biomedical Industry: In biotechnology, cell line authentication is crucial for producing vaccines, biopharmaceuticals, and other biological products. Authentic cell lines are crucial for maintaining product consistency and quality.

• Supports Regulatory Compliance: In fields subject to regulatory oversight, like clinical and pharmaceutical research, authenticated cell lines are often needed to meet regulatory compliance standards.

Why Choose DNA Forensics Laboratory Pvt. Ltd.?

DNA Forensics Laboratory Pvt. Ltd. is one of the best and most reputed DNA testing companies in India. They provide accurate, reliable, and accredited Cell Line Authentication DNA tests in India at a competitive cost. Their testing facility is accredited by NABL or National Accreditation Board for Testing & Calibration Laboratories. It is the only Indian company that offers legal DNA tests for various honorable courts of law.

They always used state-of-the-art technology and standardized methods for this testing. Their customers are from various pharmaceutical companies, reputable research institutes, biotechnology companies, etc. Moreover, they have 400+ local and international collection centers. One can visit their nearest collection center to give their cell line sample for DNA testing. They offer the cell line authentication DNA testing report within 10-15 working days.