Cell lines play a vital role in research and development, offering a cost-effective and reliable system for studying biological processes. However, a concerning issue arises due to misidentification and cross-contamination, affecting up to 36% of cell line cultures. Such errors lead to invalid research data and wasted resources, impacting scientific progress globally. To mitigate these risks, scientists can use STR DNA profiling, a simple molecular technique for human cell line authentication. Implementing routine STR DNA profiling in cell culture management can effectively detect errors and ensure accurate experimental results. By adopting this approach, researchers can safeguard the integrity of their studies, leading to more valid and meaningful scientific discoveries.
Need for a Cell Line Authentication Test in India
Three types of errors can be detected:
Cross-Contamination- Environmental contaminants like dust & fungus can easily contaminate the cells under analysis. Maintaining a pollution-free environment in the storage area and laboratory is vital to overcome this issue. Despite the care, cell contamination may be unavoidable. This test can assist the person in accurately recognizing such errors.
Misidentification- A cell line of interest needs to be identified at the source. If not detected, an expert would have continued using the same cell line for his study. They may not get the desired results. It’s a common error while working with cell lines.
Genetic Drifting- Eventually, cell lines can completely lose their identity and drift genetically into a different type of cell, affecting the test results or research.
Experts acknowledge that the latter is more destructive than the first two kinds of errors. One may be unaware that the cells are gradually changing and may continue getting inaccurate results.
How Does Cell Line Work?
Cell line authentication is conducted through STRs. Short tandem repeats are repetitive sequence elements 3-6 base pairs long and are hypervariable parts in fungi, mammals, etc. Moreover, these sequences are polymorphic, and a specific locus pattern can be repeated anytime.
The ATCC/ANSI/ASN-0002 has mentioned short tandem repeat profiling as the preferred human cell line authentication method. First, short tandem repeats are amplified by PCR using primers outside the repeat sequence. The resulting amplicons are isolated using capillary electrophoresis to specify the number of repetitions in the sample. The profile is then compared with a reference sample to prove the origin of the cell line. The statistical power of prejudice rises as a person amplifies more loci due to the repetition variation among human cell lines derived from individuals.
Why is DNA Forensics Laboratory Pvt. Ltd. the Best One?
Getting a cell line authentication DNA test in India can be a vital decision, provided the significance and sensitivity of a project. DNA Forensics Laboratory Pvt. Ltd. is among India’s best and most well-reputed DNA testing companies. They provide accurate, reliable, and accredited Cell Line Authentication DNA tests in India at a competitive cost. Their testing lab is accredited by NABL or National Accreditation Board for Testing and Calibration Laboratories. They are the only company in India that offers legal DNA tests for honorable courts of law.
They used modern technology and standardized techniques for this testing. It includes detecting the cross-contamination, misidentification, and genetically drift cells that invalidate analysis results. They have 400+ collection centers in India and abroad. One can visit the nearest collection center to give the sample for DNA testing.
Their customers are from various esteemed research institutes, biotechnology companies, pharmaceutical companies, etc. They carry out this test at their cutting-edge facility, equipped with the latest and industry-standard techniques. Furthermore, they provide the cell line authentication test report in 10-15 working days.
DNA Forensics Laboratory Pvt. Ltd. 